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KMID : 1234420110390040317
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Korean Journal of Microbiololgy and Biotechnology
2011 Volume.39 No. 4 p.317 ~ p.323
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Isolation and Characterization of Starch-hydrolyzing Pseudoalteromonas sp. A-3 from the Coastal Sea Water of Daecheon, Republic of Korea
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Chi Won-Jae
Park Da-Yeon
Hong Soon-Kwang
Jeong Sung-Cheol
Chang Yong-Keun
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Abstract
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Strain A-3, an amylase-producing bacteria, was isolated from coastal seawater near Daecheon in the Republic of Korea. It was seen to possess a single polar flagella and grow well, on ASW-YP agar plates, at temperatures of between 20-37oC. However, it grew more slowly at the temperatures of 15oC and 40oC. Similarly, it was observed to grow abundantly, in an Artificial Sea Water-Yeast extract-Peptone (ASW-YP) liquid medium, in a pH range of 6-9, but not grow at pHs of 4-5 and a pH of 10. Strain A-3 was noted as being close to Pseudoalteromonas phenolica O-BC30T, Pseudoalteromonas luteoviolacea NCIMB1893T, Pseudoalteromonas rubra ATCC29570T, and Pseudoalteromonas byunsanensis FR1199T, with 98.30%, 97.86%, 97.78%, and 97.25% similarities respectively, in its 16S rRNA sequence. A phylogenetic tree revealed that strain A-3 and P. phenolica O-BC30T belong to a clade. However, strain A-3 differed from P. phenolica O-BC30T in relation
to a number of physiological characteristics. Strain A-3 exhibited no growth above 5% NaCl concentrations, no utilization of D-glucose, D-mannose, D-maltose, or D-melibose, and no lipase (C-14) activity. All of these properties strongly indicate that strain A-3 is distant from P. phenolica O-BC30T and thus led us to name it Pseudoalteromonas sp. A-3. Pseudoalteromonas sp. A-3 produces α-amylase throughout growth. Maximal amylase activities of 144.48 U/mL and 149.20 U/mL were seen at pH 7.0 and 37oC, respectively. Pseudoalteromonas sp. A-3’s high, stable production of α-amylase in addition to its biochemical features, such as alkalitolerance, suggest that it is a good candidate for industrial applications.
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KEYWORD
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α-Amylase, marine bacteria, Pseudoalteromonas, phylogenetic analysis
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